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Living Modified Organism (LMO)
  |  
Decisions on the LMO Risk Assessments  
last updated: 15 Oct 2015
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herbicide tolerant, reduced ligning alfalfa
EN
KK179 x J101
Yes
MON-ØØ179-5 × MON-ØØ1Ø1-8
The stacked alfalfa line MON-ØØ179-5 x MON-ØØ1Ø1-8 was obtained through the traditional cross breading of each of the parental organisms to produce alfalfa that expresses the EPSPS gene and a caffeoyl CoA 3-O-methyltransferase (CCOMT) suppression cassette. The expression of these genetic elements are expected to confer tolerance to the glyphosate herbicide and reduced levels of guaiacyl lignin, respectively.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-ORGA-SCBD-12100-4 Organism Medicago sativa (Alfalfa, Lucerne, MEDSV)
    Crops
  • BCH-LMO-SCBD-15330-6 Living Modified Organism MON-ØØ1Ø1-8 - Roundup Ready™ alfalfa
    Monsanto | Resistance to herbicides (Glyphosate)
  • BCH-LMO-SCBD-105598-2 Living Modified Organism MON-ØØ179-5 - Reduced Lignin Alfalfa
    Monsanto | Changes in quality and/or metabolite content (Lignin)
EN
Characteristics of the modification process
PV-MSPQ12633 and PV-MSHT4
EN
  • Cross breeding
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-105595-1 Pal2 gene Promoter | Phaseolus vulgaris (String bean, French bean, Kidney bean, Common Bean , PHAVU)
    Promoter
  • BCH-GENE-SCBD-105596-1 Caffeoyl CoA 3-O-methyltransferase gene | Medicago sativa (Alfalfa, Lucerne, MEDSV)
    Protein coding sequence | Changes in quality and/or metabolite content (Lignin)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-101507-5 FMV 34S promoter | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)
    Promoter
  • BCH-GENE-SCBD-103901-2 HSP 70 5' untranslated leader sequence | Petunia hybrida (Petunia, PETHY)
    Leader
  • BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Transit signal
  • BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)
    Protein coding sequence | Resistance to herbicides (Glyphosate)
  • BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)
    Terminator
DNA insert from KK179 vector PV-MSPQ12633
KK179 was developed to have reduced levels of guaiacyl lignin (G). This reduction in G lignin leads to reduced accumulation of total lignin in alfalfa through the suppression of caffeoyl CoA 3-O-methyltransferase (CCOMT), a key enzyme in the lignin biosynthetic pathway through RNAi gene suppression.
The PV-MSPQ12633  vector also contains a transformation cassette expressing the nptII.

DNA insert from J101 vector PV-MSHT4
J101 contains the epsps gene from Agrobacterium tumefaciens strain CP4 to allow for the use of glyphosate, the active ingredient in the herbicide Roundup®, as a weed control option.

For additional information on this LMO, please refer to the records of the parental LMOs.
EN
LMO characteristics
EN
  • Food
  • Feed
Detection method(s)
EN
Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Risk Assessment generated by a regulatory process Living modified organism(s) 7
Country's Decision or any other Communication LMO identification 3